Chromotek gfp-trap® agarose
WebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. £198.00 - £4914.00 Specifications View More Specs Products 5 Description Specifications Description Comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads Specifications WebGeneral Plastics, Inc. 3500 North Harrison Shawnee, Oklahoma 74804. Phone: 888.275.3171. Email: [email protected]
Chromotek gfp-trap® agarose
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WebPlumber Fawn Creek KS - Local Plumbing and Emergency Plumbing Services in Fawn Creek Kansas. View. Web(gfp-trap) GFP-Trap - ChromoTek - CiteAb. 3,899 citations – An Agarose-conjugated alpaca monoclonal antibody, raised against Green fluorescent protein (Water jellyfish), supplied by ChromoTek. ... Agarose Target Green fluorescent protein (Water jellyfish) UniProt P42212 - GFP_AEQVI. Gene GFP Modification Unmodified
WebOct 19, 2024 · While anti-V5-tag antibodies for conventional IP are available from multiple suppliers, ChromoTek is the first to offer anti-V5 Nanobodies bound to agarose beads for immunoprecipitation. V5-Trap is available as agarose or magnetic agarose. Higher purity of V5-Trap vs. conventional IP No contamination with heavy or light chains using V5-Trap. WebApr 10, 2024 · Total cell extracts of yeast cells untreated or treated with CHX were incubated in ChromoTek GFP-Trap Agarose beads (Proteintech) for 2.5 h at 4 °C to pull-down AGP2-GFP tagged-associated proteins. After incubation, the beads coupled to proteins were washed with washing buffer (10 mM Tris/Cl pH 7.5, 0.5 mM EDTA, 150 …
WebDec 16, 2024 · ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ V H H conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap ® , RFP-Trap ®, and Spot-Trap ®, we offer different types of beads: Agarose beads Magnetic Agarose beads Magnetic … WebFigure Legend Snippet: a) GFP-Bub1 and GFP-bub1-kd were immunoprecipitated from mitotic (nocodazole-arrested) Cryptococcus cell extracts using GFP-trap beads, washed and added to kinase assays with or without recombinant nucleosomes as substrate. b) 6xHis-tagged, recombinant Bub1 kinase and a kinase-dead control were purified from …
WebChromoTek GmbH Christian Linke-Winnebeck ChromoTek GmbH To understand the biology of proteins and with that cell function in health and disease, it is crucial to be able to visualize proteins...
WebJan 10, 2024 · The mixture was then incubated with the GFP-Trap Agarose beads (Chromotek, pre-washed by 1 mL extraction buffer) for 4 h at 4 °C. Then, the beads were collected by spinning and washed with the extraction buffer for 3–5 times to eliminate nonspecific bindings. The bound proteins on the GFP-trap were boiled and assayed by … photos bottesWebApr 11, 2024 · A 500 μl fraction of each cleared sample was mixed with 50 μl of pre-equilibrated GFP-Trap magnetic agarose (ChromoTek Cat No. gtma) or DYKDDDDK Fab-Trap Agarose (ChromoTek Cat No. ffa) and incubated over-night at 4°C rotating end-over-end. The beads were then washed twice with Wash buffer (10 mM Tris–HCl pH 7.5; 150 … how much are walmart eggsWebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg … photos by rhonda virginia mnWebApr 5, 2024 · Bus, drive • 46h 40m. Take the bus from Miami to Houston. Take the bus from Houston Bus Station to Dallas Bus Station. Take the bus from Dallas Bus Station to … how much are war bondsWeb1. Get more from your GFP/RFP fusion Alpaca GFP-Trap ® is the top selling GFP antibody as it allows for one-step pulldowns that can be directly used in downstream applicaitons such as enzyme activity assays or mass spectrometry. 2. Best results, qualitative and quantitative GFP-/RFP trap are so easier to use than any other pull down reagent. how much are wands at universal studioshow much are war bonds worthWebJun 23, 2024 · Split GFP technologies to structurally characterize and quantify functional biomolecular interactions of FTD-related proteins. Sci Rep. 2024;7:14013 pubmed … how much are washington capitals tickets