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Psichecktm-2 vector

WebpsiCHECK™ -1 和psiCHECK™ -2 载体用于对RNA干扰(RNAi)的早期优化提供快速的定量方法。此载体可监测与报告基因融合的目的基因表达的变化。两种载体都应用海肾萤光素酶作 … Webthe PsicheckTM-2 vector to generate PsicheckTM-2-PPAR γ-WT.ThemiR-377bindingsiteinPPAR wasthenmutated,andthemutantfragmentwas 2-PPARγ-MT.Forty …

miR-151-3p Targets TWIST1 to Repress Migration of Human …

WebFeb 8, 2024 · PsiCHECKTM‐2 vector without inserted gene was used as negative control (NC) plasmid. The inserted sequences were listed in Table. Table.1. 1. Following the procedures provided by Ribobio and Promega, A7r5 cells were co‐transfected with PsiCHECKTM‐2 vector (WT/MUT/NC) and oligonucleotides (miR‐137 mimic or mimic … WebNov 18, 2015 · Downregulation of OSBP Expression by miR-124. We used the online microRNA prediction program TargetScan to investigate the potential targets of miR-124 and found four conserved miR-124 target sites in the 3′UTR of OSBP (Fig. 1a).To test whether OSBP was the direct target of miR-124, the 3′UTR of OSBP was cloned into a dual … mayerville indiana https://unitybath.com

(PDF) Hypoxia-Induced Downregulation of miR-29 in Renal

WebSep 4, 2024 · I had A vector A=[1 0 0 2 3 2 3 5 0 0 0 0 1 2 1 0 0 0 0 3 4 0 0 0 0 0 0 1 2 3 2 0 0 0 0 2 0 0] like this. Now I want a vector B having average of corresponding non ... WebMar 26, 2024 · The pGL3.0 luciferase reporter vector (E1910; Promega, Madison, WI) and psiCHECKTM-2 vector (C8021, Promega) were used for the dual-luciferase reporter assay to verify the binding sites in sequences under study for Sp1 and miR-375, respectively. WebThe 3 -UTR sequence of PPARγwas cloned and inserted into the PsicheckTM-2 vector to generate PsicheckTM- 2-PPARγ-WT.ThemiR-377bindingsiteinPPAR wasthenmutated,andthemutantfragmentwas 2-PPARγ-MT.Forty-eighthoursaftertheaforemen- tioned constructs were transfected, the luciferase activities … mayer v monroe county

MicroRNA124 Regulated Neurite Elongation by Targeting OSBP

Category:MSI、Core i9＋RTX 4080を搭載したウルトラハイエンド仕様の16 …

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Psichecktm-2 vector

Open Access Full Text Article Tumor Suppressor microRNA …

WebPromega psichecktm 2 vector Psichecktm 2 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, … WebThen, 100 ng psiCHECK‑2 luciferase reporter plasmids containing WT and M uT 3'-uTr of Bax were co-transfected into B nlcl 2 cells with mi r-214 mimic (100 nM) or mimic control (100 nM) for 48 h using ipofectaminel®2000 (Invitrogen; Thermo Fisher Scientific, Inc.).

Psichecktm-2 vector

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WebApr 11, 2024 · Vector - CAPL - CAN x 总线信息获取. 在CAN& CANFD 测试中，我们经常需要获取到CAN总线的负载、错误帧、过载帧、发送错误等等CAN总线上面的信息，这些信息如此重要，但是如果真的要写代码去实现也是相当不易的，那我们该如何去获取到的呢？. 下面我们就来一起看下 ... WebHere we demonstrate the use of the psiCHECK™-2 Vector as a biosensor for miRNA-21 (miR-21) in different cell lines and as a tool to test an miRNA target. Generating an miRNA …

WebAug 1, 2024 · The binding site of miR-509-5p to the TRAF6 3'-UTR region and identification of psiCHECKTM-2 Vector double digests. (A)The binding site of miR-509-5p to the TRAF6 3'-UTR region. Note: The underlined area indicates the target gene mutation site. (B) PCR analysis of recombinant bacteria liquid. WebShuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞. 价 格： ¥0. 货 号： 3544763. 产 地： 北京. 点击询问 我要采购. 竭诚为您服务！.

WebWelcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource … http://www.kelei-biology.com/plus/view.php?aid=1361

Web(Chuzhou, Anhui, China) and inserted into the psiCHECKTM-2 vector (Promega, Madison, WI). HEK293T cells were seeded in 24-well plates, and Lipofectamine 2000 was used to …

WebJun 17, 2024 · Example 2: Convert Matrix to Vector (sorted by rows) Using c() function. The following code shows how to convert a matrix to a vector (sorted by rows) using the c() function: #convert matrix to vector (sorted by rows) new_vector <- c(t(my_matrix)) #display vector new_vector [1] 1 6 11 16 2 7 12 17 3 8 13 18 4 9 14 19 5 10 15 20 Example 3 ... mayer vinyl tableclothWebpsiCHECK™-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in … hershorin schiff day schools of tomorrowWebNov 17, 2024 · (b) The Pax7 3’UTR with wild-type (wt) or mutant type (mut) were inserted into psiCHECK™-2 vectors and cotransfected with miR-204 mimic or negative control into HeLa cells, respectively. . (c – e) The expression of miR-204 (c), Pax7 (d), and cell division cycle genes ( CCNB (Cyclin B), CCND (Cyclin D), CCNE (Cyclin E), CDK4 (Cyclin-dependent … mayerwaren internationalWeb2.2 测序及序列对比分析 含有hsa-miR-181b结合位点及缺失hsa-miR-181b结合位点的荧光素酶报告载体psiCHECK2-TNF和psiCHECK2-TNF-mut测序结果(图3、图4)。 可见TNF的目标序列和突变序列均成功地克隆入双萤光报告载体psiCHECK2，可用于后续实验。 mayer vs carenno-busta betting tipsWebApr 9, 2024 · 作者签名：产m堤日期：2口出年‘月侈日学位论文版权使用授权书学位论文作者完全了解华中师范大学有关保留、使用学位论文的规定，即：研究生在校攻读学位期间论文工作的知识产权单位属华中师范大学。 ... －CHECK2 recombinant expressive vector carrying ... hershorin and henry llpWebinserted into psiCHECKTM-2 Vector (Promega, Madison, WI, USA) to construct plasmids used in the following dual-luciferase reporter assays. The open reading frame (ORF) of HMGB1 was cloned and inserted into pCMV6AC-GFP (Origene, - Rockville, MD, USA) vector to generate HMGB1. The primer sequences were listed in Supplementary Table 5. mayer vs alyciahttp://www.youbio.cn/product/vt1400 mayer waffen